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pcr  (New England Biolabs)


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    Structured Review

    New England Biolabs pcr
    Pcr, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ptyb21+vector/pTYB21+Vector/pm41714452-258-5-10
    Average 94 stars, based on 86 article reviews
    pcr - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Empire Assay:

    Article Title: Ion mobility mass spectrometry and molecular dynamics simulations unravel the conformational stability of human metallothionein-2 species
    Article Snippet: Tryptone, LB broth, yeast extract, isopropyl-β-D-1-thiogalactopyranoside (IPTG), and SDS were from Lab Empire, NaCl, NaOH, glycerol, KH2PO4·H2O, K2HPO4 from POCH (Gliwice Poland), pTYB21 vector and chitin resin were from New England BioLabs, and 5,5′-dithiobis-(2nitrobenzoic acid) (DTNB) from TCI Europe N.V. was purchased from Sigma-Aldrich. .. Tryptone, LB broth, yeast extract, isopropyl-β-D-1-thiogalactopyranoside (IPTG), and SDS were from Lab Empire, NaCl, NaOH, glycerol, KH2PO4·H2O, K2HPO4 from POCH (Gliwice Poland), pTYB21 vector and chitin resin were from New England BioLabs, and 5,5′-dithiobis-(2nitrobenzoic acid) (DTNB) from TCI Europe N.V. was purchased from Sigma-Aldrich. .. Expression vector (Addgene plasmid ID 105693) were transformed into BL21(DE3) E. coli cells and growth in culture medium (1.1% tryptone, 2.2% yeast extract, 0.45% glycerol, 1.3% K2HPO4, 0.38% KH2PO4) at 37°C until ~0.5 OD600.

    Article Title: Combining Native Mass Spectrometry and Proteomics to Differentiate and Map the Metalloform Landscape in Metallothioneins.
    Article Snippet: DLdithiothreitol (DTT) was purchased from Iris Biotech GmbH. .. Tryptone, LB broth, yeast extract, isopropyl-β-D-1-thiogalactopyranoside (IPTG), and SDS were from Lab Empire, NaCl, NaOH, glycerol, KH2PO4·H2O, K2HPO4 from POCH (Gliwice Poland), pTYB21 vector and chitin resin were from New England BioLabs, and 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB) from TCI Europe. ..

    Plasmid Preparation:

    Article Title: Crystal structure of the V. parahaemolyticus ToxS dimer suggests a model for bile-induced ToxRS activation
    Article Snippet: The fragment was amplified with PCR, digested with SapI and BamHI restriction enzymes, and purified using the PCR purification kit (Qiagen). .. The pTYB21 vector (NEB), containing an N-terminal chitin-binding domain intein (CBD-intein) affinity tag, was digested with the same restriction enzymes (SapI and BamHI), treated with calf intestinal phosphatase (CIP; NEB), and purified using the PCR purification kit (Qiagen) ( Table S6 ). .. The ToxSp insert was ligated into the prepared pTYB21 vector using Quick Ligase (NEB), and the ligation products were transformed into E. coli DH5α cells (NEB).

    Article Title: Molecular tension indicators reveal unexpectedly complex regulation of tension in live mouse organs
    Article Snippet: .. ActTS-GR, Sfp pet29b C-terminal His Tag, and pET28a-ybbR-His-sfGFP-DocI were purchased from Addgene (cat. 79774, 61563, 75015, and 58708), and pTYB21 vector was purchased from NEB (cat. N6709S). .. To generate TS (TS-2 in Fig. ), the coding sequence (CDS) of mutant cpEGFP within G-CAMP8 (nt 799...1521 of pN1-G-CaMP8) was cloned into pCR-Blunt II-TOPO vector (ThermoFisher, cat. 451245).

    Article Title: Differentiated Zn(II) binding affinities in animal, plant, and bacterial metallothioneins define their zinc buffering capacity at physiological pZn.
    Article Snippet: .. The cDNA encoding all investigated proteins was synthesized on request by GenScript (USA) and cloned into the pTYB21 vector using SapI and PstI (Thermo Scientific, USA) restriction sites in the case of protein Ec-1, SmtA and SpMTA or using Gibson Assembly Master Mix (New England Biolabs) for the rest of the proteins. ..

    Article Title: Molecular tension indicators reveal unexpectedly complex regulation of tension in live mouse organs.
    Article Snippet: .. ActTS-GR, Sfp pet29b C-terminal His Tag, and pET28a-ybbR-His-sfGFP-DocI were purchased from Addgene (cat. 79774, 61563, 75015, and 58708), and pTYB21 vector was purchased from NEB (cat. N6709S). .. To generate tension sensor (TS) (TS-2 in Fig.1B), the coding sequence (CDS) of mutant cpEGFP within G-CAMP8 (nt 799...1521 of pN1-G-CaMP8) was cloned into pCR-Blunt II-TOPO vector (ThermoFisher, cat. 451245).

    Article Title: Crystal structure of the V. parahaemolyticus ToxS dimer suggests a model for bile-induced ToxRS activation
    Article Snippet: The pTYB21 vector (NEB), containing an N-terminal chitin-binding domain intein (CBD-intein) affinity tag, was digested with the same restriction enzymes (SapI and BamHI), treated with calf intestinal phosphatase (CIP; NEB), and purified using the PCR purification kit (Qiagen) ( Table S6 ). .. The ToxSp insert was ligated into the prepared pTYB21 vector using Quick Ligase (NEB), and the ligation products were transformed into E. coli DH5α cells (NEB). ..

    Purification:

    Article Title: Crystal structure of the V. parahaemolyticus ToxS dimer suggests a model for bile-induced ToxRS activation
    Article Snippet: The fragment was amplified with PCR, digested with SapI and BamHI restriction enzymes, and purified using the PCR purification kit (Qiagen). .. The pTYB21 vector (NEB), containing an N-terminal chitin-binding domain intein (CBD-intein) affinity tag, was digested with the same restriction enzymes (SapI and BamHI), treated with calf intestinal phosphatase (CIP; NEB), and purified using the PCR purification kit (Qiagen) ( Table S6 ). .. The ToxSp insert was ligated into the prepared pTYB21 vector using Quick Ligase (NEB), and the ligation products were transformed into E. coli DH5α cells (NEB).

    Polymerase Chain Reaction:

    Article Title: Crystal structure of the V. parahaemolyticus ToxS dimer suggests a model for bile-induced ToxRS activation
    Article Snippet: The fragment was amplified with PCR, digested with SapI and BamHI restriction enzymes, and purified using the PCR purification kit (Qiagen). .. The pTYB21 vector (NEB), containing an N-terminal chitin-binding domain intein (CBD-intein) affinity tag, was digested with the same restriction enzymes (SapI and BamHI), treated with calf intestinal phosphatase (CIP; NEB), and purified using the PCR purification kit (Qiagen) ( Table S6 ). .. The ToxSp insert was ligated into the prepared pTYB21 vector using Quick Ligase (NEB), and the ligation products were transformed into E. coli DH5α cells (NEB).

    Synthesized:

    Article Title: Differentiated Zn(II) binding affinities in animal, plant, and bacterial metallothioneins define their zinc buffering capacity at physiological pZn.
    Article Snippet: .. The cDNA encoding all investigated proteins was synthesized on request by GenScript (USA) and cloned into the pTYB21 vector using SapI and PstI (Thermo Scientific, USA) restriction sites in the case of protein Ec-1, SmtA and SpMTA or using Gibson Assembly Master Mix (New England Biolabs) for the rest of the proteins. ..

    Clone Assay:

    Article Title: Differentiated Zn(II) binding affinities in animal, plant, and bacterial metallothioneins define their zinc buffering capacity at physiological pZn.
    Article Snippet: .. The cDNA encoding all investigated proteins was synthesized on request by GenScript (USA) and cloned into the pTYB21 vector using SapI and PstI (Thermo Scientific, USA) restriction sites in the case of protein Ec-1, SmtA and SpMTA or using Gibson Assembly Master Mix (New England Biolabs) for the rest of the proteins. ..

    Ligation:

    Article Title: Crystal structure of the V. parahaemolyticus ToxS dimer suggests a model for bile-induced ToxRS activation
    Article Snippet: The pTYB21 vector (NEB), containing an N-terminal chitin-binding domain intein (CBD-intein) affinity tag, was digested with the same restriction enzymes (SapI and BamHI), treated with calf intestinal phosphatase (CIP; NEB), and purified using the PCR purification kit (Qiagen) ( Table S6 ). .. The ToxSp insert was ligated into the prepared pTYB21 vector using Quick Ligase (NEB), and the ligation products were transformed into E. coli DH5α cells (NEB). ..

    Transformation Assay:

    Article Title: Crystal structure of the V. parahaemolyticus ToxS dimer suggests a model for bile-induced ToxRS activation
    Article Snippet: The pTYB21 vector (NEB), containing an N-terminal chitin-binding domain intein (CBD-intein) affinity tag, was digested with the same restriction enzymes (SapI and BamHI), treated with calf intestinal phosphatase (CIP; NEB), and purified using the PCR purification kit (Qiagen) ( Table S6 ). .. The ToxSp insert was ligated into the prepared pTYB21 vector using Quick Ligase (NEB), and the ligation products were transformed into E. coli DH5α cells (NEB). ..



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